Anti-OTUD7B antibody [200] (STJA0032699)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human OTUD7B protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-OTUD7B for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 200 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | OTUD7B |
| Gene ID | 56957 |
| Uniprot ID | OTU7B_HUMAN |
| Immunogen | Purified recombinant human OTUD7B protein fragments expressed in E.coli. |
Additional Info
| Tissue Specificity | Widely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells. |
| Post Translational Modifications | Phosphorylated by EGFR. |
| Function | Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B. Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2. Mediates deubiquitination of EGFR. Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro).however the physiological significance of these data are unsure. Hydrolyzes both linear and branched forms of polyubiquitin. Acts as a regulator of mTORC1 and mTORC2 assembly by mediating 'Lys-63'-linked deubiquitination of MLST8, thereby promoting assembly of the mTORC2 complex, while inibiting formation of the mTORC1 complex. |
| Protein Name | Otu Domain-Containing Protein 7bCellular Zinc Finger Anti-Nf-Kappa-B ProteinCezanneZinc Finger A20 Domain-Containing Protein 1Zinc Finger Protein Cezanne |
| Database Links | Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5689896 |
| Cellular Localisation | CytoplasmNucleusShuttles Be Cytoplasm And The Nucleus In A Xpo1/Crm1-Dependent Manner |
| Alternative Antibody Names | Anti-Otu Domain-Containing Protein 7b antibodyAnti-Cellular Zinc Finger Anti-Nf-Kappa-B Protein antibodyAnti-Cezanne antibodyAnti-Zinc Finger A20 Domain-Containing Protein 1 antibodyAnti-Zinc Finger Protein Cezanne antibodyAnti-OTUD7B antibodyAnti-ZA20D1 antibody |
Information sourced from Uniprot.org