• Immunofluorescence analysis of NIH-3T3 cells using OSBP Rabbit polyclonal antibody (STJ24872) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using OSBP antibody (STJ24872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-OSBP antibody (508-807) (STJ24872)

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STJ24872

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OSBP (508-807) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OSBP
Gene ID: 5007
Uniprot ID: OSBP1_HUMAN
Immunogen Region: 508-807
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 508-807 of human OSBP (NP_002547.1).
Immunogen Sequence: LEENGYRSLCEQVSHHPPAA AHHAESKNGWTLRQEIKITS KFRGKYLSIMPLGTIHCIFH ATGHHYTWKKVTTTVHNIIV GKLWIDQSGEIDIVNHKTGD KCNLKFVPYSYFSRDVARKV TGEVTDPSGKVHFALLGTWD EKMECFKVQPVIGENGGDAR QRGHEAEESRVMLWKRNPLP KNAENMYYFSELALTLNAWE SGTAPTDSRLRPDQRLMENG RWDEANAEKQRLEEKQRLS
Tissue Specificity Widely expressed.
Function Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
Protein Name Oxysterol-Binding Protein 1
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-192105
Cellular Localisation Cytoplasm
Cytosol
Perinuclear Region
Golgi Apparatus Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum Membrane
Golgi Apparatus
Trans-Golgi Network
Predominantly Cytosolic
Alternative Antibody Names Anti-Oxysterol-Binding Protein 1 antibody
Anti-OSBP antibody
Anti-OSBP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance