Anti-Orexin A antibody (1-50) {Biotin} (STJ502012)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide taken within amino acid region 1-50 on ORXN protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-Orexin A (1-50) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.64-0.72 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200IHC: 1:100 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Hcrt |
| Gene ID | 15171 |
| Uniprot ID | OREX_MOUSE |
| Immunogen | Synthetic peptide taken within amino acid region 1-50 on ORXN protein. |
| Immunogen Region | 1-50 |
Additional Info
| Tissue Specificity | Restricted to neuronal cell bodies of the dorsal and lateral hypothalamus. |
| Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
| Function | Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. |
| Protein Name | Hypocretin Neuropeptide PrecursorHypocretinHcrtOrexin PrecursorPrepro-OrexinPreprohypocretin Cleaved Into - Orexin-AHypocretin-1Hcrt1 - Orexin-BHypocretin-2Hcrt2 |
| Database Links | Reactome: R-MMU-389397Reactome: -MMU-416476 |
| Cellular Localisation | Rough Endoplasmic ReticulumCytoplasmic VesicleSynapseAssociated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses |
| Alternative Antibody Names | Anti-Hypocretin Neuropeptide Precursor antibodyAnti-Hypocretin antibodyAnti-Hcrt antibodyAnti-Orexin Precursor antibodyAnti-Prepro-Orexin antibodyAnti-Preprohypocretin Cleaved Into - Orexin-A antibodyAnti-Hypocretin-1 antibodyAnti-Hcrt1 - Orexin-B antibodyAnti-Hypocretin-2 antibodyAnti-Hcrt2 antibodyAnti-Hcrt antibodyAnti-Ox antibodyAnti-Ppox antibody |
Information sourced from Uniprot.org