• Immunohistochemistry analysis of paraffin-embedded human brain using ORC6 Rabbit polyclonal antibody (STJ116279) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ORC6 antibody (STJ116279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ORC6 antibody (1-252) (STJ116279)

SKU:
STJ116279

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ORC6 (1-252) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ORC6
Gene ID: 23594
Uniprot ID: ORC6_HUMAN
Immunogen Region: 1-252
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human ORC6 (NP_055136.1).
Immunogen Sequence: MGSELIGRLAPRLGLAEPDM LRKAEEYLRLSRVKCVGLSA RTTETSSAVMCLDLAASWMK CPLDRAYLIKLSGLNKETYQ SCLKSFECLLGLNSNIGIRD LAVQFSCIEAVNMASKILKS YESSLPQTQQVDLDLSRPLF TSAALLSACKILKLKVDKNK MVATSGVKKAIFDRLCKQLE KIGQQVDREPGDVATPPRKR KKIVVEAPAKEMEKVEEMPH KPQKDEDLTQDYEEWKRKI
Function Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Protein Name Origin Recognition Complex Subunit 6
Database Links Reactome: R-HSA-113507
Reactome: R-HSA-176187
Reactome: R-HSA-68616
Reactome: R-HSA-68689
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Origin Recognition Complex Subunit 6 antibody
Anti-ORC6 antibody
Anti-ORC6L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance