• Western blot analysis of extracts of various cell lines, using ORC4 antibody (STJ110018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ORC4 antibody (187-436) (STJ110018)

SKU:
STJ110018

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ORC4L (187-436) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ORC4
Gene ID: 5000
Uniprot ID: ORC4_HUMAN
Immunogen Region: 187-436
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human ORC4L (NP_002543.2).
Immunogen Sequence: IAVIGLTCRLDILELLEKRV KSRFSHRQIHLMNSFGFPQY VKIFKEQLSLPAEFPDKVFA EKWNENVQYLSEDRSVQEVL QKHFNISKNLRSLHMLLMLA LNRVTASHPFMTAVDLMEAS QLCSMDSKANIVHGLSVLEI CLIIAMKHLNDIYEEEPFNF QMVYNEFQKFVQRKAHSVYN FEKPVVMKAFEHLQQLELIK PMERTSGNSQREYQLMKLLL DNTQIMNALQKYPNCPTDV
Function Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Protein Name Origin Recognition Complex Subunit 4
Database Links Reactome: R-HSA-113507
Reactome: R-HSA-176187
Reactome: R-HSA-68616
Reactome: R-HSA-68689
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Origin Recognition Complex Subunit 4 antibody
Anti-ORC4 antibody
Anti-ORC4L antibody

Information sourced from Uniprot.org

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