Anti-ORC3 antibody (1-240) (STJ117610)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ORC3 (1-240) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ORC3 |
| Gene ID | 23595 |
| Uniprot ID | ORC3_HUMAN |
| Immunogen Region | 1-240 |
| Immunogen Sequence | MATSSMSKGCFVFKPNSKKR KISLPIEDYFNKGKNEPEDS KLRFETYQLIWQQMKSENER LQEELNKNLFDNLIEFLQKS HSGFQKNSRDLGGQIKLREI PTAALVLGVNVTDHDLTFGS LTEALQNNVTPYVVSLQAKD CPDMKHFLQKLISQLMDCCV DIKSKEEESVHVTQRKTHYS MDSLSSWYMTVTQKTDPKML SKKRTTSSQWQSPPVVVILK DMESFATKVLQDFIIISSQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ORC3 (NP_036513.2). |
Additional Info
| Post Translational Modifications | Multi-mono-ubiquitinated by OBI1.ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle. |
| Function | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3. |
| Protein Name | Origin Recognition Complex Subunit 3Origin Recognition Complex Subunit Latheo |
| Database Links | Reactome: R-HSA-113507Reactome: R-HSA-176187Reactome: R-HSA-68616Reactome: R-HSA-68689Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Origin Recognition Complex Subunit 3 antibodyAnti-Origin Recognition Complex Subunit Latheo antibodyAnti-ORC3 antibodyAnti-LATHEO antibodyAnti-ORC3L antibody |
Information sourced from Uniprot.org