• Western blot analysis of various lysates using ORC3 Rabbit polyclonal antibody (STJ117610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ORC3 antibody (1-240) (STJ117610)

SKU:
STJ117610

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ORC3 (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ORC3
Gene ID: 23595
Uniprot ID: ORC3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ORC3 (NP_036513.2).
Immunogen Sequence: MATSSMSKGCFVFKPNSKKR KISLPIEDYFNKGKNEPEDS KLRFETYQLIWQQMKSENER LQEELNKNLFDNLIEFLQKS HSGFQKNSRDLGGQIKLREI PTAALVLGVNVTDHDLTFGS LTEALQNNVTPYVVSLQAKD CPDMKHFLQKLISQLMDCCV DIKSKEEESVHVTQRKTHYS MDSLSSWYMTVTQKTDPKML SKKRTTSSQWQSPPVVVILK DMESFATKVLQDFIIISSQ
Post Translational Modifications Multi-mono-ubiquitinated by OBI1.ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle.
Function Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Protein Name Origin Recognition Complex Subunit 3
Origin Recognition Complex Subunit Latheo
Database Links Reactome: R-HSA-113507
Reactome: R-HSA-176187
Reactome: R-HSA-68616
Reactome: R-HSA-68689
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Origin Recognition Complex Subunit 3 antibody
Anti-Origin Recognition Complex Subunit Latheo antibody
Anti-ORC3 antibody
Anti-LATHEO antibody
Anti-ORC3L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance