• Western blot analysis of extracts of various cell lines, using OPA3 antibody (STJ110304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using OPA3 antibody (STJ110304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-OPA3 antibody (25-179) (STJ110304)

SKU:
STJ110304

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OPA3 (25-179) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OPA3
Gene ID: 80207
Uniprot ID: OPA3_HUMAN
Immunogen Region: 25-179
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-179 of human OPA3 (NP_079412.1).
Immunogen Sequence: NRIKEAARRSEFFKTYICLP PAQLYHWVEMRTKMRIMGFR GTVIKPLNEEAAAELGAELL GEATIFIVGGGCLVLEYWRH QAQQRHKEEEQRAAWNALRD EVGHLALALEALQAQVQAAP PQGALEELRTELQEVRAQLC NPGRSASHAVPASKK
Tissue Specificity Ubiquitous. Most prominent expression in skeletal muscle and kidney.
Function May play some role in mitochondrial processes.
Protein Name Optic Atrophy 3 Protein
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Optic Atrophy 3 Protein antibody
Anti-OPA3 antibody

Information sourced from Uniprot.org

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