• Immunohistochemistry analysis of Olig2 in paraffin-embedded mouse brain using Olig2 Rabbit polyclonal antibody (STJ114680) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Olig2 in paraffin-embedded rat brain using Olig2 Rabbit polyclonal antibody (STJ114680) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Olig2 Rabbit polyclonal antibody (STJ114680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-OLIG2 antibody (1-80) (STJ114680)

SKU:
STJ114680

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OLIG2 (1-80) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OLIG2
Gene ID: 10215
Uniprot ID: OLIG2_HUMAN
Immunogen Region: 1-80
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-80 of human Olig2 (NP_005797.1).
Immunogen Sequence: MDSDASLVSSRPSSPEPDDL FLPARSKGSSGSAFTGGTVS SSTPSDCPPELSAELRGAMG SAGAHPGDKLGGSGFKSSSS
Tissue Specificity Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Function Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Protein Name Oligodendrocyte Transcription Factor 2
Oligo2
Class B Basic Helix-Loop-Helix Protein 1
Bhlhb1
Class E Basic Helix-Loop-Helix Protein 19
Bhlhe19
Protein Kinase C-Binding Protein 2
Protein Kinase C-Binding Protein Rack17
Cellular Localisation Nucleus
Cytoplasm
The Nls Contained In The Bhlh Domain Could Be Masked In The Native Form And Translocation To The Nucleus Could Be Mediated By Interaction Either With Class E Bhlh Partner Protein Or With Nkx2-2
Alternative Antibody Names Anti-Oligodendrocyte Transcription Factor 2 antibody
Anti-Oligo2 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 1 antibody
Anti-Bhlhb1 antibody
Anti-Class E Basic Helix-Loop-Helix Protein 19 antibody
Anti-Bhlhe19 antibody
Anti-Protein Kinase C-Binding Protein 2 antibody
Anti-Protein Kinase C-Binding Protein Rack17 antibody
Anti-OLIG2 antibody
Anti-BHLHB1 antibody
Anti-BHLHE19 antibody
Anti-PRKCBP2 antibody
Anti-RACK17 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance