![Anti-OGDH antibody (869-1023) [S4736RM] (STJ11104736)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177380/369091/STJ11104736_1__20315.1713134577.jpg?c=1 "Anti-OGDH antibody (869-1023) [S4736RM] (STJ11104736)")
![Anti-OGDH antibody (869-1023) [S4736RM] (STJ11104736)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177380/369091/STJ11104736_1__20315.1713134577.jpg?c=1 "Anti-OGDH antibody (869-1023) [S4736RM] (STJ11104736)")
Anti-OGDH antibody (869-1023) [S4736RM] (STJ11104736)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal 2-Oxoglutarate Dehydrogenase Complex Component E1 (869-1023) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4736RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OGDH |
| Gene ID | 4967 |
| Uniprot ID | ODO1_HUMAN |
| Immunogen Region | 869-1023 |
| Immunogen Sequence | RSSFDEMLPGTHFQRVIPED GPAAQNPENVKRLLFCTGKV YYDLTRERKARDMVGQVAIT RIEQLSPFPFDLLLKEVQKY PNAELAWCQEEHKNQGYYDY VKPRLRTTISRAKPVWYAGR DPAAAPATGNKKTHLTELQR LLDTAFDLDVFKNFS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 869-1023 of human OGDH (NP_002532.2). |
Additional Info
| Function | 2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC. Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids. Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate. Mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. |
| Protein Name | 2-Oxoglutarate Dehydrogenase Complex Component E1E1oHsogdhOgdc-E1Ogdh-E12-Oxoglutarate Dehydrogenase - MitochondrialAlpha-Ketoglutarate DehydrogenaseAlpha-Kgdh-E1Thiamine DiphosphateThdp-Dependent 2-Oxoglutarate Dehydrogenase |
| Database Links | Reactome: R-HSA-6783984Reactome: R-HSA-9837999Reactome: R-HSA-9853506 |
| Cellular Localisation | MitochondrionNucleusMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-2-Oxoglutarate Dehydrogenase Complex Component E1 antibodyAnti-E1o antibodyAnti-Hsogdh antibodyAnti-Ogdc-E1 antibodyAnti-Ogdh-E1 antibodyAnti-2-Oxoglutarate Dehydrogenase - Mitochondrial antibodyAnti-Alpha-Ketoglutarate Dehydrogenase antibodyAnti-Alpha-Kgdh-E1 antibodyAnti-Thiamine Diphosphate antibodyAnti-Thdp-Dependent 2-Oxoglutarate Dehydrogenase antibodyAnti-OGDH antibody |
Information sourced from Uniprot.org