Anti-OGDH antibody (148-427) (STJ114351)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal OGDH (148-427) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OGDH |
| Gene ID | 4967 |
| Uniprot ID | ODO1_HUMAN |
| Immunogen Region | 148-427 |
| Immunogen Sequence | DPLGILDADLDSSVPADIIS STDKLGFYGLDESDLDKVFH LPTTTFIGGQESALPLREII RRLEMAYCQHIGVEFMFIND LEQCQWIRQKFETPGIMQFT NEEKRTLLARLVRSTRFEEF LQRKWSSEKRFGLEGCEVLI PALKTIIDKSSENGVDYVIM GMPHRGRLNVLANVIRKELE QIFCQFDSKLEAADEGSGDV KYHLGMYHRRINRVTDRNIT LSLVANPSHLEAADPVVMG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1). |
Additional Info
| Function | 2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC. Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids. Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate. Mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. |
| Protein Name | 2-Oxoglutarate Dehydrogenase Complex Component E1E1oHsogdhOgdc-E1Ogdh-E12-Oxoglutarate Dehydrogenase - MitochondrialAlpha-Ketoglutarate DehydrogenaseAlpha-Kgdh-E1Thiamine DiphosphateThdp-Dependent 2-Oxoglutarate Dehydrogenase |
| Database Links | Reactome: R-HSA-6783984Reactome: R-HSA-9837999Reactome: R-HSA-9853506 |
| Cellular Localisation | MitochondrionNucleusMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-2-Oxoglutarate Dehydrogenase Complex Component E1 antibodyAnti-E1o antibodyAnti-Hsogdh antibodyAnti-Ogdc-E1 antibodyAnti-Ogdh-E1 antibodyAnti-2-Oxoglutarate Dehydrogenase - Mitochondrial antibodyAnti-Alpha-Ketoglutarate Dehydrogenase antibodyAnti-Alpha-Kgdh-E1 antibodyAnti-Thiamine Diphosphate antibodyAnti-Thdp-Dependent 2-Oxoglutarate Dehydrogenase antibodyAnti-OGDH antibody |
Information sourced from Uniprot.org