Anti-OFD1 antibody (STJA0007287)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human OFD1
STJA0007287
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General Information

Short DescriptionRabbit polyclonal anti-Centriole and centriolar satellite protein OFD1 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolOFD1
Gene ID8481
Uniprot IDOFD1_HUMAN
ImmunogenSynthesized peptide derived from human OFD1
SpecificityThis antibody detects endogenous levels of OFD1 at Human, Mouse

Additional Info

Post Translational Modifications Phosphorylated. Phosphorylation at Ser-735, by the cAMP-dependent protein kinase PKA, triggers ubiquitination and proteasomal degradation of OFD1. Also increases its interaction with TBC1D31 and regulates its function in ciliogenesis. Ubiquitinated by PJA2, upon phosphorylation at Ser-735 by PKA, leads to the proteasomal degradation of OFD1.
Function Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation.
Protein Name Centriole And Centriolar Satellite Protein Ofd1
Oral-Facial-Digital Syndrome 1 Protein
Protein 71-7a
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5610787
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cilium Basal Body
Nucleus
Centriolar Satellite
Localizes To Centriole Distal Ends And To Centriolar Satellites
Localization To Centrioles And Pericentriolar Satellites May Be Mediated By Kiaa0753/Ofip
Alternative Antibody Names Anti-Centriole And Centriolar Satellite Protein Ofd1 antibody
Anti-Oral-Facial-Digital Syndrome 1 Protein antibody
Anti-Protein 71-7a antibody
Anti-OFD1 antibody
Anti-CXorf5 antibody

Information sourced from Uniprot.org

Citations

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