| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM13554 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB, 1:2500-1:10000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POU5F1 |
| Gene ID: | 5460 |
| Uniprot ID: | PO5F1_HUMAN |
| Immunogen Region: | 1-135aa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human OCT4 (NP_002692.2). |
| Immunogen Sequence: | Human |
| Post Translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Protein Name | Pou Domain - Class 5 - Transcription Factor 1Octamer-Binding Protein 3Oct-3Octamer-Binding Protein 4Oct-4Octamer-Binding Transcription Factor 3Otf-3 |
| Database Links | Reactome: R-HSA-2892245Reactome: R-HSA-2892247Reactome: R-HSA-452723Reactome: R-HSA-9754189Reactome: R-HSA-9823739Reactome: R-HSA-9827857Reactome: R-HSA-9834899 |
| Cellular Localisation | CytoplasmNucleusExpressed In A Diffuse And Slightly Punctuate PatternColocalizes With Mapk8 And Mapk9 In The Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 5 - Transcription Factor 1 antibodyAnti-Octamer-Binding Protein 3 antibodyAnti-Oct-3 antibodyAnti-Octamer-Binding Protein 4 antibodyAnti-Oct-4 antibodyAnti-Octamer-Binding Transcription Factor 3 antibodyAnti-Otf-3 antibodyAnti-POU5F1 antibodyAnti-OCT3 antibodyAnti-OCT4 antibodyAnti-OTF3 antibody |
Information sourced from Uniprot.org
![Anti-OCT4 antibody [MRQ-10] (STJ16100405)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/149122/346743/STJ16100405_1__87950.1681998836.jpg?c=1 "Anti-OCT4 antibody [MRQ-10] (STJ16100405)")
