Anti-OCT4/POU5F1-Mouse antibody (Internal) (STJ72238)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
General Information
| Short Description | Goat polyclonal antibody anti-OCT4/POU5F1-Mouse (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 38-40kDa band observed in lysates of cell line NIH3T3 and approx. 45kDa in lysates of cell line HepG2 (calculated MW of 38.6kDa according to Human NP_002692.2 and 38.2kDa accord |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Accession Number | NP_038661.2;Isotype=IgG" |
| Immunogen Region | Internal |
| Immunogen Sequence | DRPNAVKLEKVEP |
Additional Info
| Post Translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-106 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-228 and Ser-229 decrease DNA-binding and alters ability to activate transcription. JNK1/2-mediated phosphorylation at Ser-347 promotes proteasomal degradation. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Protein Name | Pou Domain - Class 5 - Transcription Factor 1Nf-A3Octamer-Binding Protein 3Oct-3Octamer-Binding Protein 4Oct-4Octamer-Binding Transcription Factor 3Otf-3 |
| Cellular Localisation | CytoplasmNucleusExpressed In A Diffuse And Slightly Punctuate PatternColocalizes With Mapk8 And Mapk9 In The Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 5 - Transcription Factor 1 antibodyAnti-Nf-A3 antibodyAnti-Octamer-Binding Protein 3 antibodyAnti-Oct-3 antibodyAnti-Octamer-Binding Protein 4 antibodyAnti-Oct-4 antibodyAnti-Octamer-Binding Transcription Factor 3 antibodyAnti-Otf-3 antibodyAnti-Pou5f1 antibodyAnti-Oct-3 antibodyAnti-Oct-4 antibodyAnti-Otf-3 antibodyAnti-Otf3 antibody |
Information sourced from Uniprot.org