| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000 IHC-P:1:1000-1:4000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | OCLN |
| Gene ID: | 100506658 |
| Uniprot ID: | OCLN_HUMAN |
| Immunogen Region: | 400-522 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human Occludin (NP_002529.1). |
| Immunogen Sequence: | TDYTTGGESCDELEEDWIRE YPPITSDQQRQLYKRNFDTG LQEYKSLQSELDEINKELSR LDKELDDYREESEEYMAAAD EYNRLKQVKGSADYKSKKNH CKQLKSKLSHIKKMVGDYDR QKT |
| Tissue Specificity | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. |
| Post Translational Modifications | Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB promotes bacterial translocation across the host epithelial barrier. |
| Function | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes. |
| Protein Name | Occludin |
| Database Links | Reactome: R-HSA-351906 Reactome: R-HSA-8935964 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Cell Junction Tight Junction |
| Alternative Antibody Names | Anti-Occludin antibody Anti-OCLN antibody |
Information sourced from Uniprot.org