Anti-OCLN antibody (350-450) (STJ114495)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Occludin (350-450) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OCLN |
| Gene ID | 100506658 |
| Uniprot ID | OCLN_HUMAN |
| Immunogen Region | 350-450 |
| Immunogen Sequence | VVQELPLTSPVDDFRQPRYS SGGNFETPSKRAPAKGRAGR SKRTEQDHYETDYTTGGESC DELEEDWIREYPPITSDQQR QLYKRNFDTGLQEYKSLQSE L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human Occludin (NP_002529.1). |
Additional Info
| Tissue Specificity | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. |
| Post Translational Modifications | Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB promotes bacterial translocation across the host epithelial barrier. |
| Function | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes. |
| Protein Name | Occludin |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-8935964 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionTight Junction |
| Alternative Antibody Names | Anti-Occludin antibodyAnti-OCLN antibody |
Information sourced from Uniprot.org