Anti-OBSCN antibody (1621-1710) (STJ11100081)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal OBSCN (1621-1710) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OBSCNUniprot ID=OBSCN_HUMAN" |
| Immunogen Region | 1621-1710 |
| Immunogen Sequence | EPKVVFAKEQPAHREVQAEA GASATLSCEVAQAQTEVTWY KDGKKLSSSSKVRVEAVGCT RRLVVQQAGQAEAGEYSCEA GGQQLSFRLQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1621-1710 of human OBSCN (NP_443075.3). |
Additional Info
| Post Translational Modifications | Autophosphorylated by protein kinase domains 1 and 2. |
| Function | Structural component of striated muscles which plays a role in myofibrillogenesis. Probably involved in the assembly of myosin into sarcomeric A bands in striated muscle. Has serine/threonine protein kinase activity and phosphorylates N-cadherin CDH2 and sodium/potassium-transporting ATPase subunit ATP1B1. Binds (via the PH domain) strongly to phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), and to a lesser extent to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-phosphate (PtdIns(5)P) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). |
| Protein Name | ObscurinObscurin-RhogefObscurin-Myosin Light Chain KinaseObscurin-Mlck |
| Cellular Localisation | Isoform 3: CytoplasmMyofibrilSarcomereM LineCytoplasmZ LineIn Differentiating Skeletal Muscle CellsIsoform 3 Primarily Localizes To The Sarcomeric M-Line And Less Frequently To The Z-DiskIsoform 3 Colocalizes With Ank1 Isoform Mu17/Ank15 At The M-Line In Differentiated Skeletal Muscle CellsCell MembraneSarcolemmaNucleusColocalizes With Cdh2 And Atp1b1 To The Sarcolemma And To Intercalating Disks In Cardiac MusclesColocalizes With Atp1b1 To M Line And Z Line In Cardiac Muscles |
| Alternative Antibody Names | Anti-Obscurin antibodyAnti-Obscurin-Rhogef antibodyAnti-Obscurin-Myosin Light Chain Kinase antibodyAnti-Obscurin-Mlck antibodyAnti-OBSCN antibodyAnti-KIAA1556 antibodyAnti-KIAA1639 antibody |
Information sourced from Uniprot.org