Anti-NXF1 antibody (2-50 aa) (STJ13101507)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 2-50 of mouse NXF1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat.
General Information
| Short Description | Rabbit polyclonal anti-NXF1 (2-50 aa) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Nxf1 |
| Gene ID | 53319 |
| Uniprot ID | NXF1_MOUSE |
| Immunogen | A synthetic peptide from aa region 2-50 of mouse NXF1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat. |
| Immunogen Region | 2-50 aa |
| Specificity | Specific for NXF1. |
Additional Info
| Function | Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. |
| Protein Name | Nuclear Rna Export Factor 1Tip-Associated ProteinTip-Associating ProteinMrna Export Factor Tap |
| Database Links | Reactome: R-MMU-159227Reactome: -MMU-159230Reactome: -MMU-159231Reactome: -MMU-159236 |
| Cellular Localisation | NucleusNucleoplasmNucleus SpeckleCytoplasmNuclear Pore ComplexNucleus EnvelopeStress GranuleLocalized Predominantly In The Nucleoplasm And At Both The Nucleoplasmic And Cytoplasmic Faces Of The Nuclear Pore ComplexShuttles Between The Nucleus And The CytoplasmTravels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To MrnaThe Association With The Trex Complex Seems To Occur In Regions Surrounding Nuclear Speckles Known As PerispecklesNuclear Rim |
| Alternative Antibody Names | Anti-Nuclear Rna Export Factor 1 antibodyAnti-Tip-Associated Protein antibodyAnti-Tip-Associating Protein antibodyAnti-Mrna Export Factor Tap antibodyAnti-Nxf1 antibodyAnti-Tap antibody |
Information sourced from Uniprot.org