Anti-NUP85 antibody (427-656) (STJ113234)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NUP85 (427-656) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NUP85 |
| Gene ID | 79902 |
| Uniprot ID | NUP85_HUMAN |
| Immunogen Region | 427-656 |
| Immunogen Sequence | CPELGRVSLELHIERIPLNT EQKALKVLRICEQRQMTEQV RSICKILAMKAVRNNRLGSA LSWSIRAKDAAFATLVSDRF LRDYCERGCFSDLDLIDNLG PAMMLSDRLTFLGKYREFHR MYGEKRFADAASLLLSLMTS RIAPRSFWMTLLTDALPLLE QKQVIFSAEQTYELMRCLED LTSRRPVHGESDTEQLQDDD IETTKVEMLRLSLARNLARA IIREGSLEGS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 427-656 of human NUP85 (NP_079120.1). |
Additional Info
| Function | Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Involved in nephrogenesis. |
| Protein Name | Nuclear Pore Complex Protein Nup8585 Kda NucleoporinFrountNucleoporin Nup75Nucleoporin Nup85Pericentrin-1 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-141444Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-5663220Reactome: R-HSA-6784531Reactome: R-HSA-68877Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9615933Reactome: R-HSA-9648025Reactome: R-HSA-9705671 |
| Cellular Localisation | NucleusNuclear Pore ComplexChromosomeCentromereKinetochoreCytoplasmCytoskeletonSpindleNucleus MembraneDuring MitosisLocalizes To The Kinetochores And Spindle PolesUpon Ccl2 Stimulation Translocates From The Cytoplasm To The Membrane And Colocalizes With Ccr2 At The Front Of Migrating Cells |
| Alternative Antibody Names | Anti-Nuclear Pore Complex Protein Nup85 antibodyAnti-85 Kda Nucleoporin antibodyAnti-Frount antibodyAnti-Nucleoporin Nup75 antibodyAnti-Nucleoporin Nup85 antibodyAnti-Pericentrin-1 antibodyAnti-NUP85 antibodyAnti-NUP75 antibodyAnti-PCNT1 antibody |
Information sourced from Uniprot.org