| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-NuMA (2000-2100) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S7MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NUMA1 |
| Gene ID: |
4926 |
| Uniprot ID: |
NUMA1_HUMAN |
| Immunogen Region: |
2000-2100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 2000-2100 of human NUMA11 (Q14980). |
| Immunogen Sequence: |
TPESKKATSCFPRPMTPRDR HEGRKQSTTEAQKKAAPAST KQADRRQSMAFSILNTPKKL GNSLLRRGASKKALSKASPN TRSGTRRSPRIATTTASAAT A |
| Post Translational Modifications | Phosphorylation and dephosphorylation on Thr-2055 regulates the extent of cortical NUMA1 and the dynein-dynactin complex localization during mitotic metaphase and anaphase. In metaphase, phosphorylation on Thr-2055 occurs in a kinase CDK1-dependent manner.this phosphorylation maintains low levels of cortical dynein-dynactin complex at metaphase, and hence proper spindle positioning. In anaphase, dephosphorylated on Thr-2055 by phosphatase PPP2CA.this dephosphorylation stimulates its membrane association and with the dynein-dynactin complex its enrichment at the cell cortex, and hence robust spindle elongation. Probably also phosphorylated on Thr-2015 and Ser-2087 by CDK1.these phosphorylations may regulate its cell cortex recruitment during metaphase and anaphase. Phosphorylated on Thr-1047, Ser-1769, Ser-1772, Ser-1789 and Ser-1834 by PLK1.these phosphorylations induce cortical dynein-dynactin complex dissociation from the NUMA1-GPSM2 complex and negatively regulates cortical dynein-dynactin complex localization. ADP-ribosylated by TNKS at the onset of mitosis.ADP-ribosylation is not required for its localization to spindle poles. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. Ubiquitinated with 'Lys-63'-linked polyubiquitin chains. Deubiquitination by the BRISC complex is important for the incorporation of NUMA1 into mitotic spindle poles and normal spindle pole function, probably by modulating interactions between NUMA1, dynein-dynactin complex and importin-beta. |
| Function | Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume. Required for epidermal differentiation and hair follicle morphogenesis. |
| Protein Name | Nuclear Mitotic Apparatus Protein 1Nuclear Matrix Protein-22Nmp-22Nuclear Mitotic Apparatus ProteinNuma ProteinSp-H Antigen |
| Database Links | Reactome: R-HSA-380320Reactome: R-HSA-68875 |
| Cellular Localisation | NucleusNucleoplasmNucleus MatrixChromosomeCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle PoleCell CortexCell MembraneLipid-AnchorCytoplasmic SideLateral Cell MembraneMitotic Cell Cycle-Dependent Shuttling Protein That Relocalizes From The Interphase Nucleus To The Spindle Poles And Cell CortexThe Localization To The Spindle Poles Is Regulated By AaasIn InterphaseResides In The Nuclear MatrixIn ProphaseRestricted To The Interchromatin Or Condensed Chromosome SpaceIn PrometaphaseAfter Nuclear Envelope DisassemblyForms Aggregates Both In The Spindle Midzone And At Duplicated Centrosomes And Astral Microtubules (Mts) Of The Bipolar Spindle ApparatusTranslocates From The Spindle Midzone Towards The Spindle Poles Along Spindle Fibers In A Mt- And Dynein-Dynactin-Dependent Manner Until The Anaphase OnsetIn MetaphaseRecruited To The Polar Cortical Region In A Gpsm2- And Gnai1-Dependent MannerExcluded From The Metaphase Equatorial Cortical Region In A Rangtp-Dependent MannerPhosphorylation On Thr-2055 By Cdk1 Results In Its Localization At Spindle Poles In MetaphaseBut Not At The Cell CortexIn AnaphaseRecruited And Anchored At The Cell Membrane Of The Polar Cortical Region In A Epb41-Epb41l2-Phosphatidylinositol-Dependent And Gpsm2- And G(I) Alpha Proteins-Independent MannerExcluded From The Anaphase Equatorial Region Of The Cell Cortex In A Racgap1- And Kif23-Dependent And Rangtp-Independent MannerAssociated With Astral Mts Emanating From The Spindle Poles During AnaphaseNonphosphorylated Thr-2055 Localizes At The Cell CortexWeakly During Metaphase And More Prominently During Anaphase In A Phosphatase Ppp2ca-Dependent MannerAs Mitosis Progresses It Reassociates With Telophase Chromosomes Very Early During Nuclear ReformationBefore Substantial Accumulation Of Lamins On Chromosomal Surfaces Is EvidentLocalizes To The Tips Of Cortical Mts In PrometaphaseLocalizes Along Mts And Specifically To Both Mt Plus And Minus EndsAccumulates Also At Mt Tips Near The Cell PeripheryColocalizes With Gpsm2 At Mitotic Spindle Poles During MitosisColocalizes With Spag5 At Mitotic Spindle At Prometaphase And At Mitotic Spindle Poles At Metaphase And AnaphaseColocalizes With Abro1 At Mitotic Spindle PolesColocalized With Tnks From Prophase Through To Anaphase In MitosisColocalizes With Tubulin AlphaCcsap Is Essential For Its Centrosomal LocalizationIn Horizontally Retinal Progenitor Dividing CellsLocalized To The Lateral Cortical RegionIsoform 3: CytoplasmCytosolDuring InterphaseMainly Clustered At The Centrosomal Region In The CytosolAfter Entry Into MitosisDetected At Mitotic Spindle PolesIsoform 4: Cytoplasm |
| Alternative Antibody Names | Anti-Nuclear Mitotic Apparatus Protein 1 antibodyAnti-Nuclear Matrix Protein-22 antibodyAnti-Nmp-22 antibodyAnti-Nuclear Mitotic Apparatus Protein antibodyAnti-Numa Protein antibodyAnti-Sp-H Antigen antibodyAnti-NUMA1 antibodyAnti-NMP22 antibodyAnti-NUMA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
