Anti-NUDT21 antibody (1-227) (STJ24845)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NUDT21 (1-227) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NUDT21 |
| Gene ID | 11051 |
| Uniprot ID | CPSF5_HUMAN |
| Immunogen Region | 1-227 |
| Immunogen Sequence | MSVVPPNRSQTGWPRGVTQF GNKYIQQTKPLTLERTINLY PLTNYTFGTKEPLYEKDSSV AARFQRMREEFDKIGMRRTV EGVLIVHEHRLPHVLLLQLG TTFFKLPGGELNPGEDEVEG LKRLMTEILGRQDGVLQDWV IDDCIGNWWRPNFEPPQYPY IPAHITKPKEHKKLFLVQLQ EKALFAVPKNYKLVAAPLFE LYDNAPGYGPIISSLPQLLS RFNFIYN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human NUDT21 (NP_008937.1). |
Additional Info
| Tissue Specificity | Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 and SIRT2. |
| Function | Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. |
| Protein Name | Cleavage And Polyadenylation Specificity Factor Subunit 5Cleavage And Polyadenylation Specificity Factor 25 Kda SubunitCpsf 25 Kda SubunitCleavage Factor Im Complex 25 Kda SubunitCfim25Nucleoside Diphosphate-Linked Moiety X Motif 21Nudix Motif 21Nudix Hydrolase 21Pre-Mrna Cleavage Factor Im 68 Kda Subunit |
| Database Links | Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856Reactome: R-HSA-77595 |
| Cellular Localisation | NucleusCytoplasmShuttles Between The Nucleus And The Cytoplasm In A Transcription- And Xpo1/Crm1-Independent MannerMost Probably In Complex With The Cleavage Factor Im Complex (Cfim)In Punctate Subnuclear Structures Localized Adjacent To Nuclear SpecklesCalled Paraspeckles |
| Alternative Antibody Names | Anti-Cleavage And Polyadenylation Specificity Factor Subunit 5 antibodyAnti-Cleavage And Polyadenylation Specificity Factor 25 Kda Subunit antibodyAnti-Cpsf 25 Kda Subunit antibodyAnti-Cleavage Factor Im Complex 25 Kda Subunit antibodyAnti-Cfim25 antibodyAnti-Nucleoside Diphosphate-Linked Moiety X Motif 21 antibodyAnti-Nudix Motif 21 antibodyAnti-Nudix Hydrolase 21 antibodyAnti-Pre-Mrna Cleavage Factor Im 68 Kda Subunit antibodyAnti-NUDT21 antibodyAnti-CFIM25 antibodyAnti-CPSF25 antibodyAnti-CPSF5 antibody |
Information sourced from Uniprot.org