• Western blot analysis of extracts of various cell lines, using NUDT15 antibody (STJ110666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using NUDT15 antibody (STJ110666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using NUDT15 antibody (STJ110666) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver using NUDT15 antibody (STJ110666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using NUDT15 antibody (STJ110666) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using NUDT15 antibody (STJ110666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NUDT15 antibody (1-164) (STJ110666)

SKU:
STJ110666

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUDT15 (1-164) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUDT15
Gene ID: 55270
Uniprot ID: NUD15_HUMAN
Immunogen Region: 1-164
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human NUDT15 (NP_060753.1).
Immunogen Sequence: MTASAQPRGRRPGVGVGVVV TSCKHPRCVLLGKRKGSVGA GSFQLPGGHLEFGETWEECA QRETWEEAALHLKNVHFASV VNSFIEKENYHYVTILMKGE VDVTHDSEPKNVEPEKNESW EWVPWEELPPLDQLFWGLRC LKEQGYDPFKEDLNHLVGYK GNHL
Function May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP. Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives. Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear. Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs. May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
Protein Name Nucleotide Triphosphate Diphosphatase Nudt15
Mutt Homolog 2
Mth2
Nucleoside Diphosphate-Linked Moiety X Motif 15
Nudix Motif 15
Nucleoside Diphosphate-Linked To Another Moiety X Hydrolase 15
Nudix Hydrolase 15
Database Links Reactome: R-HSA-2393930
Reactome: R-HSA-9748787
Alternative Antibody Names Anti-Nucleotide Triphosphate Diphosphatase Nudt15 antibody
Anti-Mutt Homolog 2 antibody
Anti-Mth2 antibody
Anti-Nucleoside Diphosphate-Linked Moiety X Motif 15 antibody
Anti-Nudix Motif 15 antibody
Anti-Nucleoside Diphosphate-Linked To Another Moiety X Hydrolase 15 antibody
Anti-Nudix Hydrolase 15 antibody
Anti-NUDT15 antibody
Anti-MTH2 antibody

Information sourced from Uniprot.org

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