• Western blot analysis of extracts of various cell lines, using NUDC antibody (STJ28761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NUDC antibody (1-331) (STJ28761)

SKU:
STJ28761

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUDC (1-331) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUDC
Gene ID: 10726
Uniprot ID: NUDC_HUMAN
Immunogen Region: 1-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human NUDC (NP_006591.1).
Immunogen Sequence: MGGEQEEERFDGMLLAMAQQ HEGGVQELVNTFFSFLRRKT DFFIGGEEGMAEKLITQTFS HHNQLAQKTRREKRARQEAE RREKAERAARLAKEAKSETS GPQIKELTDEEAERLQLEID QKKDAENHEAQLKNGSLDSP GKQDTEEDEEEDEKDKGKLK PNLGNGADLPNYRWTQTLSE LDLAVPFCVNFRLKGKDMVV DIQRRHLRVGLKGQPAIIDG ELYNEVKVEESSWLIEDGK
Tissue Specificity Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.
Post Translational Modifications Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases.
Function Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
Protein Name Nuclear Migration Protein Nudc
Nuclear Distribution Protein C Homolog
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Reactome: R-HSA-9696270
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Spindle
Midbody
In A Filamentous Pattern Adjacent To The Nucleus Of Migrating Cerebellar Granule Cells
Colocalizes With Tubulin And Dynein And With The Microtubule Organizing Center
Distributed Throughout The Cytoplasm Of Non-Migrating Cells
A Small Proportion Is Nuclear
In A Punctate Pattern
Localizes To The Mitotic Spindle In A Eml4-Dependent Manner
Alternative Antibody Names Anti-Nuclear Migration Protein Nudc antibody
Anti-Nuclear Distribution Protein C Homolog antibody
Anti-NUDC antibody

Information sourced from Uniprot.org

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