| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Trk B (C-Term) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was antigen affinity purified from serum. |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Ntrk2 |
| Gene ID: |
25054 |
| Uniprot ID: |
NTRK2_RAT |
| Immunogen Region: |
C-Term |
| Immunogen: |
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. |
| Tissue Specificity | Widely expressed in the central and peripheral nervous system. The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons. |
| Post Translational Modifications | Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Some isoforms are not phosphorylated. Ubiquitinated. Undergoes polyubiquitination upon activation.regulated by NGFR. Ubiquitination regulates the internalization of the receptor. |
| Function | Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells. |
| Protein Name | Bdnf/Nt-3 Growth Factors ReceptorNeurotrophic Tyrosine Kinase Receptor Type 2Trkb Tyrosine KinaseTrk-B |
| Database Links | Reactome: R-RNO-9026527Reactome: -RNO-9028731Reactome: -RNO-9032759 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndosome MembraneEarly Endosome MembraneCell ProjectionAxonDendriteCytoplasmPerinuclear RegionPostsynaptic DensityInternalized To Endosomes Upon Ligand-Binding |
| Alternative Antibody Names | Anti-Bdnf/Nt-3 Growth Factors Receptor antibodyAnti-Neurotrophic Tyrosine Kinase Receptor Type 2 antibodyAnti-Trkb Tyrosine Kinase antibodyAnti-Trk-B antibodyAnti-Ntrk2 antibodyAnti-Trkb antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
