• Immunohistochemistry analysis of paraffin-embedded human liver damage using NTF3 antibody (STJ114350) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using NTF3 antibody (STJ114350) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NTF3 antibody (STJ114350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NTF3 antibody (19-257) (STJ114350)

SKU:
STJ114350

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NTF3 (19-257) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NTF3
Gene ID: 4908
Uniprot ID: NTF3_HUMAN
Immunogen Region: 19-257
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-257 of human NTF3 (NP_002518.1).
Immunogen Sequence: NNMDQRSLPEDSLNSLIIKL IQADILKNKLSKQMVDVKEN YQSTLPKAEAPREPERGGPA KSAFQPVIAMDTELLRQQRR YNSPRVLLSDSTPLEPPPLY LMEDYVGSPVVANRTSRRKR YAEHKSHRGEYSVCDSESLW VTDKSSAIDIRGHQVTVLGE IKTGNSPVKQYFYETRCKEA RPVKNGCRGIDDKHWNSQCK TSQTYVRALTSENNKLVGWR WIRIDTSCVCALSRKIGRT
Tissue Specificity Brain and peripheral tissues.
Function Seems to promote the survival of visceral and proprioceptive sensory neurons.
Protein Name Neurotrophin-3
Nt-3
Hdnf
Nerve Growth Factor 2
Ngf-2
Neurotrophic Factor
Database Links Reactome: R-HSA-9025046
Reactome: R-HSA-9034013
Reactome: R-HSA-9034015
Reactome: R-HSA-9034793
Reactome: R-HSA-9034864
Reactome: R-HSA-9603381
Cellular Localisation Secreted
Alternative Antibody Names Anti-Neurotrophin-3 antibody
Anti-Nt-3 antibody
Anti-Hdnf antibody
Anti-Nerve Growth Factor 2 antibody
Anti-Ngf-2 antibody
Anti-Neurotrophic Factor antibody
Anti-NTF3 antibody

Information sourced from Uniprot.org

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