![Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100179/386325/STJ24827_1__72801.1713155370.jpg?c=1 "Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human liver cancer using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100179/386326/STJ24827_2__11785.1713155371.jpg?c=1 "Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human liver cancer using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and CD73/NT5E knockout (KO) HCT116 cells, using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100179/386327/STJ24827_3__62996.1713155371.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD73/NT5E knockout (KO) HCT116 cells, using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from Rat eye, using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100179/386328/STJ24827_4__50626.1713155372.jpg?c=1 "Western blot analysis of lysates from Rat eye, using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100179/386329/STJ24827_5__84085.1713155373.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/100179/386325/STJ24827_1__72801.1713155370.jpg?c=1 "Immunohistochemistry analysis of CD73/NT5E in paraffin-embedded human tonsil using [KO Validated] CD73/NT5E Rabbit polyclonal antibody (STJ24827) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
Anti-NT5E antibody (27-574) (STJ24827)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NT5E/CD73 (27-574) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:3000-1:30000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NT5E |
| Gene ID | 4907 |
| Uniprot ID | 5NTD_HUMAN |
| Immunogen Region | 27-574 |
| Immunogen Sequence | WELTILHTNDVHSRLEQTSE DSSKCVNASRCMGGVARLFT KVQQIRRAEPNVLLLDAGDQ YQGTIWFTVYKGAEVAHFMN ALRYDAMALGNHEFDNGVEG LIEPLLKEAKFPILSANIKA KGPLASQISGLYLPYKVLPV GDEVVGIVGYTSKETPFLSN PGTNLVFEDEITALQPEVDK LKTLNVNKIIALGHSGFEMD KLIAQKVRGVDVVVGGHSNT FLYTGNPPSKEVPAGKYPF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 27-377 of human CD73/NT5E (NP_002517.1). |
Additional Info
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. |
| Protein Name | 5'-Nucleotidase5'-Nt 5'-DeoxynucleotidaseEcto-5'-NucleotidaseImp-Specific 5'-NucleotidaseThymidylate 5'-PhosphataseCd Antigen Cd73 |
| Database Links | Reactome: R-HSA-196807Reactome: R-HSA-73621Reactome: R-HSA-74259Reactome: R-HSA-9660826 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-5'-Nucleotidase antibodyAnti-5'-Nt antibodyAnti-5'-Deoxynucleotidase antibodyAnti-Ecto-5'-Nucleotidase antibodyAnti-Imp-Specific 5'-Nucleotidase antibodyAnti-Thymidylate 5'-Phosphatase antibodyAnti-Cd Antigen Cd73 antibodyAnti-NT5E antibodyAnti-NT5 antibodyAnti-NTE antibody |
Information sourced from Uniprot.org