• Immunohistochemistry analysis of paraffin-embedded Rat brain using NSUN5 antibody ( STJ11100922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using NSUN5 antibody ( STJ11100922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NSUN5 antibody ( STJ11100922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NSUN5 antibody (STJ11100922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat brain, using NSUN5 antibody (STJ11100922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NSUN5 antibody (127-429) (STJ11100922)

SKU:
STJ11100922

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSUN5 (127-429) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSUN5
Gene ID: 55695
Uniprot ID: NSUN5_HUMAN
Immunogen Region: 127-429
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-429 of human NSUN5 (NP_060514.1).
Immunogen Sequence: RPGPASQLPRFVRVNTLKTC SDDVVDYFKRQGFSYQGRAS SLDDLRALKGKHFLLDPLMP ELLVFPAQTDLHEHPLYRAG HLILQDRASCLPAMLLDPPP GSHVIDACAAPGNKTSHLAA LLKNQGKIFAFDLDAKRLAS MATLLARAGVSCCELAEEDF LAVSPSDPRYHEVHYILLDP SCSGSGMPSRQLEEPGAGTP SPVRLHALAGFQQRALCHAL TFPSLQRLVYSTCSLCQEE
Tissue Specificity Ubiquitous. Detected in placenta, heart and skeletal muscle.
Function S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA. m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity. Required for corpus callosum and cerebral cortex development.
Protein Name 28s Rrna
Cytosine-C(5-Methyltransferase
Nol1-Related Protein
Nol1r
Nol1/Nop2/Sun Domain Family Member 5
Williams-Beuren Syndrome Chromosomal Region 20a Protein
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-28s Rrna antibody
Anti-Cytosine-C(5-Methyltransferase antibody
Anti-Nol1-Related Protein antibody
Anti-Nol1r antibody
Anti-Nol1/Nop2/Sun Domain Family Member 5 antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 20a Protein antibody
Anti-NSUN5 antibody
Anti-NSUN5A antibody
Anti-WBSCR20 antibody
Anti-WBSCR20A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance