Anti-NSMCE2 antibody (STJA0008288)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human NSMCE2
General Information
| Short Description | Rabbit polyclonal anti-E3 SUMO-protein ligase NSE2 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NSMCE2 |
| Gene ID | 286053 |
| Uniprot ID | NSE2_HUMAN |
| Immunogen | Synthesized peptide derived from human NSMCE2 |
| Specificity | This antibody detects endogenous levels of NSMCE2 at Human, Mouse, Rat |
Additional Info
| Function | E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression. |
| Protein Name | E3 Sumo-Protein Ligase Nse2E3 Sumo-Protein Transferase Nse2Mms21 HomologHmms21Non-Structural Maintenance Of Chromosomes Element 2 HomologNon-Smc Element 2 Homolog |
| Database Links | Reactome: R-HSA-3108214 |
| Cellular Localisation | NucleusChromosomeTelomerePml BodyLocalizes To Pml Nuclear Bodies In Alt Cell Lines |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Nse2 antibodyAnti-E3 Sumo-Protein Transferase Nse2 antibodyAnti-Mms21 Homolog antibodyAnti-Hmms21 antibodyAnti-Non-Structural Maintenance Of Chromosomes Element 2 Homolog antibodyAnti-Non-Smc Element 2 Homolog antibodyAnti-NSMCE2 antibodyAnti-C8orf36 antibodyAnti-MMS21 antibody |
Information sourced from Uniprot.org