| Tissue Specificity | Detected in keratinocytes, liver and placenta. Expressed in a subset of cells in pediatric medulloblastoma. |
| Function | May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro). Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy. Stabilizes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3. Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1. May be involved in autophagosome biogenesis. May play a role in neural progenitor cell survival during differentiation. |
| Protein Name | Nuclear Receptor-Binding Factor 2Nrbf-2Comodulator Of Ppar And Rxr |
| Database Links | Reactome: R-HSA-383280 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic VesicleAutophagosome |
| Alternative Antibody Names | Anti-Nuclear Receptor-Binding Factor 2 antibodyAnti-Nrbf-2 antibodyAnti-Comodulator Of Ppar And Rxr antibodyAnti-NRBF2 antibodyAnti-COPR antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NRBF2 knockout (KO) HeLa cells, using [KO Validated] NRBF2 Rabbit polyclonal antibody (STJ11100732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89670/358165/STJ11100732_1__69868.1713122469.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NRBF2 knockout (KO) HeLa cells, using [KO Validated] NRBF2 Rabbit polyclonal antibody (STJ11100732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and NRBF2 knockout (KO) HeLa cells, using [KO Validated] NRBF2 Rabbit polyclonal antibody (STJ11100732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89670/358165/STJ11100732_1__69868.1713122469.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NRBF2 knockout (KO) HeLa cells, using [KO Validated] NRBF2 Rabbit polyclonal antibody (STJ11100732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
