• Western blot analysis of lysates from wild type (WT) and NRBF2 knockout (KO) HeLa cells, using [KO Validated] NRBF2 Rabbit polyclonal antibody (STJ11100732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NRBF2 antibody (10-220) (STJ11100732)

SKU:
STJ11100732

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NRBF2 (10-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRBF2
Gene ID: 29982
Uniprot ID: NRBF2_HUMAN
Immunogen Region: 10-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-220 of human NRBF2 (NP_110386.2).
Immunogen Sequence: LAHQQSRRADRLLAAGKYEE AISCHKKAAAYLSEAMKLTQ SEQAHLSLELQRDSHMKQLL LIQERWKRAQREERLKAQQN TDKDAAAHLQTSHKPSAEDA EGQSPLSQKYSPSTEKCLPE IQGIFDRDPDTLLYLLQQKS EPAEPCIGSKAPKDDKTIIE EQATKIADLKRHVEFLVAEN ERLRKENKQLKAEKARLLKG PIEKELDVDAD
Tissue Specificity Detected in keratinocytes, liver and placenta. Expressed in a subset of cells in pediatric medulloblastoma.
Function May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro). Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy. Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3. Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1. May be involved in autophagosome biogenesis. May play a role in neural progenitor cell survival during differentiation.
Protein Name Nuclear Receptor-Binding Factor 2
Nrbf-2
Comodulator Of Ppar And Rxr
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Vesicle
Autophagosome
Alternative Antibody Names Anti-Nuclear Receptor-Binding Factor 2 antibody
Anti-Nrbf-2 antibody
Anti-Comodulator Of Ppar And Rxr antibody
Anti-NRBF2 antibody
Anti-COPR antibody

Information sourced from Uniprot.org

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