• Western blot analysis of extracts of HepG2 cells, using NRBF2 antibody (STJ28545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts from normal (control) and NRBF2 knockout (KO) 293T cells, using NRBF2 antibody (STJ28545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using [KO Validated] NRBF2 rabbit polyclonal antibody (STJ28545) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using NRBF2 antibody (STJ28545) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using NRBF2 antibody (STJ28545) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using NRBF2 antibody (STJ28545) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using NRBF2 rabbit polyclonal antibody (STJ28545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using NRBF2 rabbit polyclonal antibody (STJ28545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using NRBF2 rabbit polyclonal antibody (STJ28545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NRBF2 antibody (1-287) (STJ28545)

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STJ28545

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NRBF2 (1-287) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRBF2
Gene ID: 29982
Uniprot ID: NRBF2_HUMAN
Immunogen Region: 1-287
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NRBF2 (NP_110386.2).
Immunogen Sequence: MEVMEGPLNLAHQQSRRADR LLAAGKYEEAISCHKKAAAY LSEAMKLTQSEQAHLSLELQ RDSHMKQLLLIQERWKRAQR EERLKAQQNTDKDAAAHLQT SHKPSAEDAEGQSPLSQKYS PSTEKCLPEIQGIFDRDPDT LLYLLQQKSEPAEPCIGSKA PKDDKTIIEEQATKIADLKR HVEFLVAENERLRKENKQLK AEKARLLKGPIEKELDVDAD FVETSELWSLPPHAETATA
Tissue Specificity Detected in keratinocytes, liver and placenta. Expressed in a subset of cells in pediatric medulloblastoma.
Function May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro). Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy. Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3. Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1. May be involved in autophagosome biogenesis. May play a role in neural progenitor cell survival during differentiation.
Protein Name Nuclear Receptor-Binding Factor 2
Nrbf-2
Comodulator Of Ppar And Rxr
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Vesicle
Autophagosome
Alternative Antibody Names Anti-Nuclear Receptor-Binding Factor 2 antibody
Anti-Nrbf-2 antibody
Anti-Comodulator Of Ppar And Rxr antibody
Anti-NRBF2 antibody
Anti-COPR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance