• Immunofluorescence analysis of MCF7 cells using NR2C1 antibody (STJ28758).
  • Western blot analysis of extracts of various cell lines, using NR2C1 antibody (STJ28758) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-NR2C1 antibody (STJ28758)

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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against NR2C1.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IF: 1:50-1:200
Uniprot ID: NR2C1_HUMAN
Gene ID: 7181
Gene Symbol: NR2C1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NR2C1 (NP_003288.2).
Background This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD) , a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.
Post Translational Modifications Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression. Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB.
Function Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Pml Body
Recruited By Hdac3
After All-Trans Retinoic Acid Stimulated Mapk1-Mediated Thr-223 Phosphorylation
To Pml Bodies For Subsequent Sumoylation
Protein Name Nuclear Receptor Subfamily 2 Group C Member 1
Orphan Nuclear Receptor Tr2
Testicular Receptor 2
Alternative Names Anti-Nuclear Receptor Subfamily 2 Group C Member 1 antibody
Anti-Orphan Nuclear Receptor Tr2 antibody
Anti-Testicular Receptor 2 antibody
Anti-NR2C1 antibody
Anti-TR2 antibody

Information sourced from uniprot.org

6 months standard