• Immunofluorescence analysis of HepG2 cells using NR1I3 Rabbit polyclonal antibody (STJ11104529) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse ovary, using NR1I3 Rabbit polyclonal antibody (STJ11104529) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NR1I3 antibody (99-352) (STJ11104529)

SKU:
STJ11104529

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR1I3
Gene ID: 9970
Uniprot ID: NR1I3_HUMAN
Immunogen Region: 99-352
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 99-352 of human NR1I3 (NP_001070948.1).
Immunogen Sequence: AQQTPVQLSKEQEELIRTLL GAHTRHMGTMFEQFVQFRPP AHLFIHHQPLPTLAPVLPLV THFADINTFMVLQVIKFTKD LPVFRSLPIEDQISLLKGAA VEICHIVLNTTFCLQTQNFL CGPLRYTIEDGARVSPTVGF QVEFLELLFHFHGTLRKLQL QEPEYVLLAAMALFSPDRPG VTQRDEIDQLQEEMALTLQS YIKGQQRRPRDRFLYAKLLG LLAELRSINEAYGYQIQHI
Tissue Specificity Predominantly expressed in liver.
Post Translational Modifications Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.
Function Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
Protein Name Nuclear Receptor Subfamily 1 Group I Member 3
Constitutive Activator Of Retinoid Response
Constitutive Active Response
Constitutive Androstane Receptor
Car
Orphan Nuclear Receptor Mb67
Database Links Reactome: R-HSA-383280
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Recruited To The Cytoplasm By Dnajc7
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 1 Group I Member 3 antibody
Anti-Constitutive Activator Of Retinoid Response antibody
Anti-Constitutive Active Response antibody
Anti-Constitutive Androstane Receptor antibody
Anti-Car antibody
Anti-Orphan Nuclear Receptor Mb67 antibody
Anti-NR1I3 antibody
Anti-CAR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance