• STJ70143 staining (0. 1µg/ml) of U251 cell lysate (RIPA buffer, 35µg total protein per lane). Detected using chemiluminescence.
  • STJ70143 staining (0. 03µg/ml) of Human Kidney (A) and (0. 3ug/ml) Lung (B) lysate (RIPA buffer, [35µg total protein per lane). Detected using chemiluminescence.
  • STJ70143 (1µg/ml) staining of Rat (A) and Pig (B) Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
  • STJ70143 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70143 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-NQO1 antibody (C-Term) (STJ70143)

SKU:
STJ70143-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Rat/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NQO1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HepG2 and U251 cells. 5-10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NQO1
Gene ID: 1728
Uniprot ID: NQO1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000894.1; NP_001020604.1; NP_001020605.1
Specificity: This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).
Immunogen Sequence: SIPTDNQIKARK
Protein Name Nad(Ph Dehydrogenase Quinone 1
Azoreductase
Dt-Diaphorase
Dtd
Menadione Reductase
Nad(Ph -Quinone Oxidoreductase 1
Phylloquinone Reductase
Quinone Reductase 1
Qr1
Database Links Reactome: R-HSA-350562
Reactome: R-HSA-9818027
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Nad(Ph Dehydrogenase Quinone 1 antibody
Anti-Azoreductase antibody
Anti-Dt-Diaphorase antibody
Anti-Dtd antibody
Anti-Menadione Reductase antibody
Anti-Nad(Ph -Quinone Oxidoreductase 1 antibody
Anti-Phylloquinone Reductase antibody
Anti-Quinone Reductase 1 antibody
Anti-Qr1 antibody
Anti-NQO1 antibody
Anti-DIA4 antibody
Anti-NMOR1 antibody

Information sourced from Uniprot.org

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