• Immunohistochemistry analysis of paraffin-embedded rat heart using NPPA Rabbit polyclonal antibody (STJ116955) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using NPPA Rabbit polyclonal antibody (STJ116955) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse heart, using NPPA antibody (STJ116955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-NPPA antibody (26-151) (STJ116955)

SKU:
STJ116955

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NPPA (26-151) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPPA
Gene ID: 4878
Uniprot ID: ANF_HUMAN
Immunogen Region: 26-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-151 of human NPPA (NP_006154.1).
Immunogen Sequence: LTWQEIMSITELQGLNAPSE PSFEPQAPAPYLGPPPPTTY CPCSIHPDSGFPLPPPPYEL PASTSHVPDPPYSYGNMAIP VSKPLSLSGLLSEPLQDPLA LLDIGLPAGPPKPQEDPESD SGLSLN
Tissue Specificity Urodilatin: Detected in the kidney distal tubular cells (at protein level). Present in urine (at protein level). Atrial natriuretic peptide: Detected in atrial and ventricular plasma samples, and in adipocytes (at protein level). Detected in urine in one study. However, was not detected in urine in another study. In the brain, predominantly expressed in the gray matter with very weak expression in the white matter (at protein level). Localizes to astrocyte-like structures throughout the white matter, and in the cerebral vessels detected in the leptomeningeal and parenchymal vessels, and endothelium and smooth muscle layers (at protein level). Relatively low levels of expression in the kidneys compared to urodilatin (at protein level).
Post Translational Modifications The precursor molecule is proteolytically cleaved by CORIN at Arg-123 to produce atrial natriuretic peptide. Undergoes further proteolytic cleavage by unknown proteases to give rise to long-acting natriuretic peptide, vessel dilator and kaliuretic peptide. Additional processing gives rise to the auriculin and atriopeptin peptides. In the kidneys, alternative processing by an unknown protease results in the peptide urodilatin. Atrial natriuretic peptide: Cleavage by MME initiates degradation of the factor and thereby regulates its activity. Degraded by IDE (in vitro). During IDE degradation, the resulting products can temporarily stimulate NPR2 to produce cGMP, before the fragments are completely degraded and inactivated by IDE (in vitro). Urodilatin: Degraded by IDE. Urodilatin: Phosphorylation on Ser-129 decreases vasorelaxant activity.
Function Atrial natriuretic peptide: Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses. Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance. Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts. Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension. In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis. This includes up-regulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue. Binds the clearance receptor NPR3 which removes the hormone from circulation. Long-acting natriuretic peptide: May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, vasodilation, and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting.according to a report, in vivo it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by atrial natriuretic peptide and vessel dilator. Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption. Vessel dilator: May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, and vasodilation. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting.according to a report it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by the atrial natriuretic and long-acting natriuretic peptides. Possibly functions in protein excretion in urine by maintaining the integrity of the proximal tubules and enhancing protein excretion by decreasing proximal tubular protein reabsorption. Kaliuretic peptide: May have a role in cardio-renal homeostasis through regulation of diuresis and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. May have a role in potassium excretion but not sodium excretion (natriuresis). Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption. Urodilatin: Hormone produced in the kidneys that appears to be important for maintaining cardio-renal homeostasis. Mediates vasodilation, natriuresis and diuresis primarily in the renal system, in order to maintain the extracellular fluid volume and control the fluid-electrolyte balance. Specifically binds and stimulates cGMP production by renal transmembrane receptors, likely NPR1. Urodilatin not ANP, may be the natriuretic peptide responsible for the regulation of sodium and water homeostasis in the kidney. Auriculin-D: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-B: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips. Auriculin-A: May have a role in cardio-renal homeostasis through regulation of regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, vasodilates intestinal smooth muscle but not smooth muscle strips. Atriopeptin-2: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal and vascular smooth muscle strips. Atriopeptin-1: May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal smooth muscle but not vascular smooth muscle strips.
Protein Name Natriuretic Peptides A
Atrial Natriuretic Factor Prohormone
Proanf
Atrial Natriuretic Peptide Prohormone
Preproanp
Proanp
Atriopeptigen
Cardiodilatin
Cdd
Preprocdd-Anf Cleaved Into - Long-Acting Natriuretic Peptide
Lanp
Long-Acting Natriuretic Hormone
Lanh
Pro Atrial Natriuretic Factor 1-30
Proanf 1-30
Pro Atrial Natriuretic Peptide 1-30
Proanp 1-30 - Vessel Dilator
Vsdl
Pro Atrial Natriuretic Factor 31-67
Proanf 31-67
Pro Atrial Natriuretic Peptide 31-67
Proanp 31-67 - Kaliuretic Peptide
Kp
Pro Atrial Natriuretic Factor 79-98
Proanf 79-98
Pro Atrial Natriuretic Peptide 79-98
Proanp 79-98 - Urodilatin
Uro
Cdd 95-126
Cdd-Anp
95-126
Pro Atrial Natriuretic Peptide 95-126
Proanp 95-126 - Auriculin-C
Atrial Natriuretic Factor 1-33
Anf 1-33 - Auriculin-D
Atrial Natriuretic Factor 3-33
Anf 3-33 - Atrial Natriuretic Peptide
Anp
Alpha-Atrial Natriuretic Peptide
Alpha-Hanp
Atrial Natriuretic Factor
Anf
Cdd-Anf
Cdd-Anp
99-126
Cardionatrin
Pro Atrial Natriuretic Factor 99-126
Proanf 99-126 - Auriculin-B
Atrial Natriuretic Factor 8-33
Anf 8-33 - Auriculin-A - Atriopeptin-1
Atriopeptin I - Atriopeptin-2
Atriopeptin Ii - Atriopeptin-3
Atriopeptin Iii
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-5578768
Reactome: R-HSA-977225
Cellular Localisation Long-Acting Natriuretic Peptide: Secreted
Detected In Blood
Vessel Dilator: Secreted
Kaliuretic Peptide: Secreted
Urodilatin: Secreted
Detected In Urine
Not Detected In Blood
Increased Electrolytes
Osmolality And Intracellular Camp Levels Increase Peptide Secretion/Excretion
Atrial Natriuretic Peptide: Secreted
Perikaryon
Cell Projection
Detected In Urine In One Study
However
In Another Study
Was Not Detected In Urine
Detected In Cytoplasmic Bodies And Neuronal Processes Of Pyramidal Neurons (Layers Ii-Vi)
Increased Secretion In Response To The Vasopressin Avp
Likely To Be Secreted In Response To An Increase In Atrial Pressure Or Atrial Stretch
In Kidney Cells
Secretion Increases In Response To Activated Guanylyl Cyclases And Increased Intracellular Camp Levels
Plasma Levels Increase 15 Minutes After A High-Salt Meal
And Decrease Back To Normal Plasma Levels 1 Hr Later
Atriopeptin-3: Secreted
Slight Increase In Secretion In Response To The Vasopressin Avp
Alternative Antibody Names Anti-Natriuretic Peptides A antibody
Anti-Atrial Natriuretic Factor Prohormone antibody
Anti-Proanf antibody
Anti-Atrial Natriuretic Peptide Prohormone antibody
Anti-Preproanp antibody
Anti-Proanp antibody
Anti-Atriopeptigen antibody
Anti-Cardiodilatin antibody
Anti-Cdd antibody
Anti-Preprocdd-Anf Cleaved Into - Long-Acting Natriuretic Peptide antibody
Anti-Lanp antibody
Anti-Long-Acting Natriuretic Hormone antibody
Anti-Lanh antibody
Anti-Pro Atrial Natriuretic Factor 1-30 antibody
Anti-Proanf 1-30 antibody
Anti-Pro Atrial Natriuretic Peptide 1-30 antibody
Anti-Proanp 1-30 - Vessel Dilator antibody
Anti-Vsdl antibody
Anti-Pro Atrial Natriuretic Factor 31-67 antibody
Anti-Proanf 31-67 antibody
Anti-Pro Atrial Natriuretic Peptide 31-67 antibody
Anti-Proanp 31-67 - Kaliuretic Peptide antibody
Anti-Kp antibody
Anti-Pro Atrial Natriuretic Factor 79-98 antibody
Anti-Proanf 79-98 antibody
Anti-Pro Atrial Natriuretic Peptide 79-98 antibody
Anti-Proanp 79-98 - Urodilatin antibody
Anti-Uro antibody
Anti-Cdd 95-126 antibody
Anti-Cdd-Anp antibody
Anti-95-126 antibody
Anti-Pro Atrial Natriuretic Peptide 95-126 antibody
Anti-Proanp 95-126 - Auriculin-C antibody
Anti-Atrial Natriuretic Factor 1-33 antibody
Anti-Anf 1-33 - Auriculin-D antibody
Anti-Atrial Natriuretic Factor 3-33 antibody
Anti-Anf 3-33 - Atrial Natriuretic Peptide antibody
Anti-Anp antibody
Anti-Alpha-Atrial Natriuretic Peptide antibody
Anti-Alpha-Hanp antibody
Anti-Atrial Natriuretic Factor antibody
Anti-Anf antibody
Anti-Cdd-Anf antibody
Anti-Cdd-Anp antibody
Anti-99-126 antibody
Anti-Cardionatrin antibody
Anti-Pro Atrial Natriuretic Factor 99-126 antibody
Anti-Proanf 99-126 - Auriculin-B antibody
Anti-Atrial Natriuretic Factor 8-33 antibody
Anti-Anf 8-33 - Auriculin-A - Atriopeptin-1 antibody
Anti-Atriopeptin I - Atriopeptin-2 antibody
Anti-Atriopeptin Ii - Atriopeptin-3 antibody
Anti-Atriopeptin Iii antibody
Anti-NPPA antibody
Anti-ANP antibody
Anti-PND antibody

Information sourced from Uniprot.org

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