Anti-NPL antibody (1-250) (STJ113623)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NPL (1-250) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NPL |
| Gene ID | 80896 |
| Uniprot ID | NPL_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MAFPKKKLQGLVAATITPMT ENGEINFSVIGQYVDYLVKE QGVKNIFVNGTTGEGLSLSV SERRQVAEEWVTKGKDKLDQ VIIHVGALSLKESQELAQHA AEIGADGIAVIAPFFLKPWT KDILINFLKEVAAAAPALPF YYYHIPALTGVKIRAEELLD GILDKIPTFQGLKFSDTDLL DFGQCVDQNRQQQFAFLFGV DEQLLSALVMGATGAVGSTY NYLGKKTNQMLEAFEQKDF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NPL (NP_110396.1). |
Additional Info
| Tissue Specificity | Isoform 2 is expressed in placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine and peripheral blood leukocyte. |
| Function | Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (Probable). |
| Protein Name | N-Acetylneuraminate LyaseNalaseN-Acetylneuraminate Pyruvate-LyaseN-Acetylneuraminic Acid AldolaseSialate LyaseSialate-Pyruvate LyaseSialic Acid AldolaseSialic Acid Lyase |
| Database Links | Reactome: R-HSA-4085001 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-N-Acetylneuraminate Lyase antibodyAnti-Nalase antibodyAnti-N-Acetylneuraminate Pyruvate-Lyase antibodyAnti-N-Acetylneuraminic Acid Aldolase antibodyAnti-Sialate Lyase antibodyAnti-Sialate-Pyruvate Lyase antibodyAnti-Sialic Acid Aldolase antibodyAnti-Sialic Acid Lyase antibodyAnti-NPL antibodyAnti-C1orf13 antibody |
Information sourced from Uniprot.org