• Western blot analysis of various lysates, using NPHS2 antibody (STJ119463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-NPHS2 antibody (300-388) (STJ119463)

SKU:
STJ119463

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NPHS2 (300-388) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPHS2
Gene ID: 7827
Uniprot ID: PODO_HUMAN
Immunogen Region: 300-388
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-388 of human NPHS2 (NP_055440.1).
Immunogen Sequence: AASESLRMAAEILSGTPAAV QLRYLHTLQSLSTEKPSTVV LPLPFDLLNCLSSPSNRTQG SLPFPSPSKPVEPLNPKKKD SPML
Tissue Specificity Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
Post Translational Modifications Isoform 2: Glycosylated.
Function Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Protein Name Podocin
Database Links Reactome: R-HSA-373753
Cellular Localisation Isoform 1: Cell Membrane
Peripheral Membrane Protein
Isoform 2: Endoplasmic Reticulum
Alternative Antibody Names Anti-Podocin antibody
Anti-NPHS2 antibody

Information sourced from Uniprot.org

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