Anti-NPHS1 antibody (843-892) (STJ94401)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human Nephrin. AA range:843-892
General Information
| Short Description | Rabbit polyclonal anti-Nephrin (843-892) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NPHS1 |
| Gene ID | 4868 |
| Uniprot ID | NPHN_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Nephrin. AA range:843-892 |
| Immunogen Region | 843-892 |
| Specificity | Nephrin Polyclonal Antibody detects endogenous levels of Nephrin protein. |
Additional Info
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli. |
| Post Translational Modifications | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-HSA-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPredominantly Located At Podocyte Slit Diaphragm Between Podocyte Foot ProcessesAlso Associated With Podocyte Apical Plasma Membrane |
| Alternative Antibody Names | Anti-Nephrin antibodyAnti-Renal Glomerulus-Specific Cell Adhesion Receptor antibodyAnti-NPHS1 antibodyAnti-NPHN antibody |
Information sourced from Uniprot.org