Anti-NPC2 antibody (20-151) (STJ27366)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NPC2 (20-151) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NPC2 |
| Gene ID | 10577 |
| Uniprot ID | NPC2_HUMAN |
| Immunogen Region | 20-151 |
| Immunogen Sequence | EPVQFKDCGSVDGVIKEVNV SPCPTQPCQLSKGQSYSVNV TFTSNIQSKSSKAVVHGILM GVPVPFPIPEPDGCKSGINC PIQKDKTYSYLNKLPVKSEY PSIKLVVEWQLQDDKNQSLF CWEIPVQIVSHL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 20-151 of human NPC2 (NP_006423.1). |
Additional Info
| Tissue Specificity | Detected in gallbladder bile. Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level). Epididymis. |
| Function | Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport. |
| Protein Name | Npc Intracellular Cholesterol Transporter 2Epididymal Secretory Protein E1Human Epididymis-Specific Protein 1He1Niemann-Pick Disease Type C2 Protein |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-8964038 |
| Cellular Localisation | SecretedEndoplasmic ReticulumLysosomeInteraction With Cell-Surface M6pr Mediates Endocytosis And Targeting To Lysosomes |
| Alternative Antibody Names | Anti-Npc Intracellular Cholesterol Transporter 2 antibodyAnti-Epididymal Secretory Protein E1 antibodyAnti-Human Epididymis-Specific Protein 1 antibodyAnti-He1 antibodyAnti-Niemann-Pick Disease Type C2 Protein antibodyAnti-NPC2 antibodyAnti-HE1 antibody |
Information sourced from Uniprot.org