| Host: | Goat |
| Applications: | Pep-ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Npc1 (mouse)-Mouse (Internal) is suitable for use in ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in th |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: | Internal |
| Accession Number: | NP_032746.2 |
| Immunogen Sequence: | TYHTILKTSADYTD |
| Post Translational Modifications | N-glycosylated. |
| Function | Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). |
| Protein Name | Npc Intracellular Cholesterol Transporter 1Niemann-Pick C1 Protein |
| Database Links | Reactome: R-MMU-8964038 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLysosome Membrane |
| Alternative Antibody Names | Anti-Npc Intracellular Cholesterol Transporter 1 antibodyAnti-Niemann-Pick C1 Protein antibodyAnti-Npc1 antibody |
Information sourced from Uniprot.org
