| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-Npap60/Nup50 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | WB-Recommended for use at 0.2-1µg/mlIHC-Recommended concentration, 2.5-3.8µg/mlELISA-antibody detection limit dilution 1:32000. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NUP50 |
| Gene ID: | 10762 |
| Uniprot ID: | NUP50_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_009103.2; NP_705931.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1). |
| Immunogen Sequence: | ELHKILLEKKDA |
| Function | Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. |
| Protein Name | Nuclear Pore Complex Protein Nup5050 Kda NucleoporinNuclear Pore-Associated Protein 60 Kda-LikeNucleoporin Nup50 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-6784531Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9705671 |
| Cellular Localisation | NucleusNuclear Pore ComplexNucleus MembranePeripheral Membrane ProteinNucleoplasmic SideLocalizes To The Nucleoplasmic Fibrils Of The Nuclear Pore ComplexDissociates From The Npc Structure Early During Prophase Of MitosisAssociates With The Newly Formed Nuclear Membrane During TelophaseIn The TestisThe Localization Changes During Germ Cell Differentiation From The Nuclear Surface In Spermatocytes To The Whole Nucleus (Interior) In Spermatids And Back To The Nuclear Surface In Spermatozoa |
| Alternative Antibody Names | Anti-Nuclear Pore Complex Protein Nup50 antibodyAnti-50 Kda Nucleoporin antibodyAnti-Nuclear Pore-Associated Protein 60 Kda-Like antibodyAnti-Nucleoporin Nup50 antibodyAnti-NUP50 antibodyAnti-NPAP60L antibodyAnti-PRO1146 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
