Anti-NOXO1 antibody (C-Term) (STJ70517)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70517-100
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General Information

Short DescriptionGoat polyclonal antibody anti-NOXO1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications.
ApplicationsPep-ELISA/IHC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
WB: Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1µg/ml.
IHC: In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolNOXO1
Gene ID124056
Uniprot IDNOXO1_HUMAN
Accession NumberNP_653204.1; NP_751907.1; NP_751907.1
Immunogen RegionC-Term
Immunogen SequenceRGCVDSVPHPTTEQ
SpecificityThis antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).

Additional Info

Function Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.
Protein Name Nadph Oxidase Organizer 1
Nadph Oxidase Regulatory Protein
Nox Organizer 1
Nox-Organizing Protein 1
Sh3 And Px Domain-Containing Protein 5
Database Links Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Reactome: R-HSA-9673324
Cellular Localisation Isoform 3: Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Isoform 3 Associates With The Plasma Membrane In A Lipid-Dependent Manner
Alternative Antibody Names Anti-Nadph Oxidase Organizer 1 antibody
Anti-Nadph Oxidase Regulatory Protein antibody
Anti-Nox Organizer 1 antibody
Anti-Nox-Organizing Protein 1 antibody
Anti-Sh3 And Px Domain-Containing Protein 5 antibody
Anti-NOXO1 antibody
Anti-P41NOX antibody
Anti-SH3PXD5 antibody

Information sourced from Uniprot.org

Citations

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