Anti-NOVA1 antibody (Internal) (STJ71492)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-NOVA1 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Applications | Pep-ELISA/WB/IHC/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2). Recommended concen |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | NOVA1 |
| Gene ID | 4857 |
| Uniprot ID | NOVA1_HUMAN |
| Accession Number | NP_002506.2 ; NP_006480.2 ; NP_006482.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | REMPQNVAKTEPVS |
| Specificity | This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1) |
Additional Info
| Function | Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord. |
| Protein Name | Rna-Binding Protein Nova-1Neuro-Oncological Ventral Antigen 1Onconeural Ventral Antigen 1Paraneoplastic Ri AntigenVentral Neuron-Specific Protein 1 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Rna-Binding Protein Nova-1 antibodyAnti-Neuro-Oncological Ventral Antigen 1 antibodyAnti-Onconeural Ventral Antigen 1 antibodyAnti-Paraneoplastic Ri Antigen antibodyAnti-Ventral Neuron-Specific Protein 1 antibodyAnti-NOVA1 antibody |
Information sourced from Uniprot.org