• STJ70825 (4µg/ml) staining of paraffin embedded Human Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70825 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
  • STJ70825 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-NOTCH3 antibody (C-Term) (STJ70825)

SKU:
STJ70825-100

£303.50
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Host: Goat
Applications: Pep-ELISA/FC/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NOTCH3 (C-Term) is suitable for use in ELISA, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-4-6µg/ml, 5µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NOTCH3
Gene ID: 4854
Uniprot ID: NOTC3_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000426.2
Immunogen Sequence: QLGPQPEVTPKRQ
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN.
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Protein Name Neurogenic Locus Notch Homolog Protein 3
Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-9013507
Reactome: R-HSA-9013508
Reactome: R-HSA-9017802
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 3 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Alternative Antibody Names Anti-Neurogenic Locus Notch Homolog Protein 3 antibody
Anti-Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain antibody
Anti-NOTCH3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance