Anti-NOTCH2/3 antibody [59R1] {FITC} (STJA0028554)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationFITC
IsotypeIgG1k
General Information
| Short Description | Rat monoclonal NOTCH2/3 antibody for use in ELISA and FC in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/FC |
| Host | Rat |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 59R1 |
| Isotype | IgG1k |
| Conjugation | FITC |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Formulation | PBS pH 7.4, 50% glycerol, 0.05% proclin300 |
| Storage Instruction | Store at 4°C for 12 months. Protect from light. Do not freeze. |
Target Information
| Gene Symbol | Notch2 |
| Gene ID | 18129 |
| Uniprot ID | NOTC2_MOUSE |
Additional Info
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Hydroxylated by HIF1AN. Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1. Phosphorylated by GSK3. GSK3-mediated phosphorylation is necessary for NOTCH2 recognition by FBXW7, ubiquitination and degradation via the ubiquitin proteasome pathway. |
| Function | Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 2Notch 2Motch B Cleaved Into - Notch 2 Extracellular Truncation - Notch 2 Intracellular Domain |
| Database Links | |
| Cellular Localisation | Notch 2 Extracellular Truncation: Cell MembraneSingle-Pass Type I Membrane ProteinNotch 2 Intracellular Domain: NucleusCytoplasmFollowing Proteolytical Processing Nicd Is Translocated To The NucleusRetained At The Cytoplasm By Tcim |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 2 antibodyAnti-Notch 2 antibodyAnti-Motch B Cleaved Into - Notch 2 Extracellular Truncation - Notch 2 Intracellular Domain antibodyAnti-Notch2 antibody |
Information sourced from Uniprot.org