• Immunofluorescence analysis of U-2 OS cells using NOSTRIN Rabbit polyclonal antibody (STJ118383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NOSTRIN Rabbit polyclonal antibody (STJ118383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NOSTRIN antibody (270-430) (STJ118383)

SKU:
STJ118383

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOSTRIN (270-430) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOSTRIN
Gene ID: 115677
Uniprot ID: NOSTN_HUMAN
Immunogen Region: 270-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-430 of human NOSTRIN (NP_001034813.2).
Immunogen Sequence: ILSTENKSEFLLTDYFEEDP NSAMDKERRKSLLKPKLLRL QRDIEKASKDKEGLERMLKT YSSTSSFSDAKSQKDTAALM DENNLKLDLLEANSYKLSSM LAELEQRPQPSHPCSNSIFR WREKEHTHSYVKISRPFLMK RLENIVSKASSGGQSNPGSS T
Tissue Specificity Expressed at highest levels in heart, kidney, placenta and lung, and at lowest levels in brain, thymus and spleen. Present in vascular endothelial cells and placenta. Over-expressed in placenta from women with pre-eclampsia (at protein level).
Function Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.
Protein Name Nostrin
Bm247 Homolog
Nitric Oxide Synthase Traffic Inducer
Nitric Oxide Synthase Trafficker
Enos-Trafficking Inducer
Database Links Reactome: R-HSA-203641
Cellular Localisation Isoform 1: Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Cytoplasm
Cytoskeleton
Enriched In Selected Actin Structures
Isoform 3: Nucleus
Alternative Antibody Names Anti-Nostrin antibody
Anti-Bm247 Homolog antibody
Anti-Nitric Oxide Synthase Traffic Inducer antibody
Anti-Nitric Oxide Synthase Trafficker antibody
Anti-Enos-Trafficking Inducer antibody
Anti-NOSTRIN antibody

Information sourced from Uniprot.org

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