Anti-NOP58 antibody (360-529) (STJ24780)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NOP58 (360-529) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NOP58 |
| Gene ID | 51602 |
| Uniprot ID | NOP58_HUMAN |
| Immunogen Region | 360-529 |
| Immunogen Sequence | RMLAAKTVLAIRYDAFGEDS SSAMGVENRAKLEARLRTLE DRGIRKISGTGKALAKTEKY EHKSEVKTYDPSGDSTLPTC SKKRKIEQVDKEDEITEKKA KKAKIKVKVEEEEEEKVAEE EETSVKKKKKRGKKKHIKEE PLSEEEPCTSTAIASPEKKK KKKKKRENED |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 360-529 of human NOP58 (NP_057018.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Sumoylation is essential for high-affinity binding to snoRNAs. |
| Function | Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs). |
| Protein Name | Nucleolar Protein 58Nucleolar Protein 5 |
| Database Links | Reactome: R-HSA-4570464Reactome: R-HSA-6790901Reactome: R-HSA-6791226 |
| Cellular Localisation | NucleusNucleolusNucleoplasmLocalizes To The Nucleolus With A Minor Part Present In The Nucleoplasm |
| Alternative Antibody Names | Anti-Nucleolar Protein 58 antibodyAnti-Nucleolar Protein 5 antibodyAnti-NOP58 antibodyAnti-NOL5 antibodyAnti-NOP5 antibodyAnti-HSPC120 antibody |
Information sourced from Uniprot.org