• Immunofluorescence analysis of U-2 OS cells using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using NONO/p54nrb Rabbit polyclonal antibody (STJ27235) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NONO/p54nrb antibody (STJ27235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NONO antibody (1-471) (STJ27235)

SKU:
STJ27235

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NONO (1-471) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NONO
Gene ID: 4841
Uniprot ID: NONO_HUMAN
Immunogen Region: 1-471
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-471 of human NONO/p54nrb (NP_001138881.1).
Immunogen Sequence: MQSNKTFNLEKQNHTPRKHH QHHHQQQHHQQQQQQPPPPP IPANGQQASSQNEGLTIDLK NFRKPGEKTFTQRSRLFVGN LPPDITEEEMRKLFEKYGKA GEVFIHKDKGFGFIRLETRT LAEIAKVELDNMPLRGKQLR VRFACHSASLTVRNLPQYVS NELLEEAFSVFGQVERAVVI VDDRGRPSGKGIVEFSGKPA ARKALDRCSEGSFLLTTFPR PVTVEPMDQLDDEEGLPEK
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
Post Translational Modifications The N-terminus is blocked.
Function DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. Promotes activation of the cGAS-STING pathway in response to HIV-2 infection: acts by interacting with HIV-2 Capsid protein p24, thereby promoting detection of viral DNA by CGAS, leading to CGAS-mediated inmmune activation. In contrast, the weak interaction with HIV-1 Capsid protein p24 does not allow activation of the cGAS-STING pathway.
Protein Name Non-Pou Domain-Containing Octamer-Binding Protein
Nono Protein
54 Kda Nuclear Rna- And Dna-Binding Protein
P54(Nrb
P54nrb
55 Kda Nuclear Protein
Nmt55
Dna-Binding P52/P100 Complex - 52 Kda Subunit
Cellular Localisation Nucleus
Nucleolus
Nucleus Speckle
Chromosome
Detected In Punctate Subnuclear Structures Often Located Adjacent To Splicing Speckles
Called Paraspeckles
Alternative Antibody Names Anti-Non-Pou Domain-Containing Octamer-Binding Protein antibody
Anti-Nono Protein antibody
Anti-54 Kda Nuclear Rna- And Dna-Binding Protein antibody
Anti-P54(Nrb antibody
Anti-P54nrb antibody
Anti-55 Kda Nuclear Protein antibody
Anti-Nmt55 antibody
Anti-Dna-Binding P52/P100 Complex - 52 Kda Subunit antibody
Anti-NONO antibody
Anti-NRB54 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance