Anti-NOLC1 antibody [R03-7F1] (STJA0034649)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human NOLC1
General Information
| Short Description | Rabbit monoclonal anti-NOLC1 for use in WB, IHC-F, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-7F1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | NOLC1 |
| Gene ID | 9221 |
| Uniprot ID | NOLC1_HUMAN |
| Immunogen | A synthetic peptide of human NOLC1 |
Additional Info
| Post Translational Modifications | Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell. Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Pyrophosphorylated by 5-diphosphoinositol pentakisphosphate (5-IP7). Serine pyrophosphorylation is achieved by Mg(2+)-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue. |
| Function | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. |
| Protein Name | Nucleolar And Coiled-Body Phosphoprotein 1140 Kda Nucleolar PhosphoproteinNopp140Hepatitis C Virus Ns5a-Transactivated Protein 13Hcv Ns5a-Transactivated Protein 13Nucleolar 130 Kda ProteinNucleolar Phosphoprotein P130 |
| Database Links | |
| Cellular Localisation | NucleusNucleolusCytoplasmShuttles Between The Nucleolus And The CytoplasmAt Telophase It Begins To Assemble Into Granular-Like Pre-Nucleolar Bodies Which Are Subsequently Relocated To Nucleoli At The Early G1-Phase |
| Alternative Antibody Names | Anti-Nucleolar And Coiled-Body Phosphoprotein 1 antibodyAnti-140 Kda Nucleolar Phosphoprotein antibodyAnti-Nopp140 antibodyAnti-Hepatitis C Virus Ns5a-Transactivated Protein 13 antibodyAnti-Hcv Ns5a-Transactivated Protein 13 antibodyAnti-Nucleolar 130 Kda Protein antibodyAnti-Nucleolar Phosphoprotein P130 antibodyAnti-NOLC1 antibodyAnti-KIAA0035 antibodyAnti-NS5ATP13 antibody |
Information sourced from Uniprot.org