• Immunohistochemistry analysis of paraffin-embedded mouse kidney using NOD1 antibody (STJ24778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using NOD1 antibody (STJ24778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using NOD1 antibody (STJ24778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NOD1 antibody (STJ24778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-NOD1 antibody (1-270) (STJ24778)

SKU:
STJ24778

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOD1 (1-270) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOD1
Gene ID: 10392
Uniprot ID: NOD1_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NOD1 (NP_006083.1).
Immunogen Sequence: MEEQGHSEMEIIPSESHPHI QLLKSNRELLVTHIRNTQCL VDNLLKNDYFSAEDAEIVCA CPTQPDKVRKILDLVQSKGE EVSEFFLYLLQQLADAYVDL RPWLLEIGFSPSLLTQSKVV VNTDPVSRYTQQLRHHLGRD SKFVLCYAQKEELLLEEIYM DTIMELVGFSNESLGSLNSL ACLLDHTTGILNEQGETIFI LGDAGVGKSMLLQRLQSLWA TGRLDAGVKFFFHFRCRMF
Tissue Specificity Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
Post Translational Modifications Palmitoylated. Palmitoylation is required for proper recruitment to the bacterial entry site and hence for proper signaling upon cognate peptidoglycan detection. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activation. Degraded via selective autophagy following interaction with IRGM. IRGM promotes NOD1-RIPK2 RIPosome recruitment to autophagosome membranes, promoting their SQSTM1/p62-dependent autophagic degradation.
Function Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses. Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria. Preferentially binds iE-DAP in tripeptide-containing muropeptides (MurNAc-TriDAP or TriDAP). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2. Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Also acts as a regulator of antiviral response elicited by dsRNA and the expression of RLR pathway members by targeting IFIH1 and TRAF3 to modulate the formation of IFIH1-MAVS and TRAF3-MAVS complexes leading to increased transcription of type I IFNs. Also acts as a regulator of autophagy via its interaction with ATG16L1, possibly by recruiting ATG16L1 at the site of bacterial entry. Besides recognizing pathogens, also involved in the endoplasmic reticulum stress response: acts by sensing and binding to the cytosolic metabolite sphingosine-1-phosphate generated in response to endoplasmic reticulum stress, initiating an inflammation process that leads to activation of the NF-kappa-B and MAP kinases signaling. In addition, plays a role in insulin trafficking in beta cells in a cell-autonomous manner. Mechanistically, upon recognizing cognate ligands, NOD1 and RIPK2 localize to insulin vesicles where they recruit RAB1A to direct insulin trafficking through the cytoplasm. Isoform 3: In contrast to isoform 1, does not efficiently recognize and bind gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP) ligand.
Protein Name Nucleotide-Binding Oligomerization Domain-Containing Protein 1
Hnod1
Caspase Recruitment Domain-Containing Protein 4
Database Links Reactome: R-HSA-168638
Reactome: R-HSA-445989
Reactome: R-HSA-450302
Reactome: R-HSA-450321
Reactome: R-HSA-5689896
Reactome: R-HSA-9020702
Reactome: R-HSA-9705671
Cellular Localisation Cell Membrane
Lipid-Anchor
Apical Cell Membrane
Basolateral Cell Membrane
Cytoplasm
Detected In The Cytoplasm And At The Cell Membrane
Following Bacterial Infection
Localizes To Bacterial Entry Sites In The Cell Membrane
Recruited To The Basolateral And Apical Membranes In Polarized Epithelial Cells
Alternative Antibody Names Anti-Nucleotide-Binding Oligomerization Domain-Containing Protein 1 antibody
Anti-Hnod1 antibody
Anti-Caspase Recruitment Domain-Containing Protein 4 antibody
Anti-NOD1 antibody
Anti-CARD4 antibody

Information sourced from Uniprot.org

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