• Immunofluorescence analysis of U-2 OS cells using NOB1 Rabbit polyclonal antibody (STJ119733) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using NOB1 Rabbit polyclonal antibody (STJ119733) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using NOB1 Rabbit polyclonal antibody (STJ119733) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NOB1 Rabbit polyclonal antibody (STJ119733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NOB1 antibody (1-230) (STJ119733)

SKU:
STJ119733

$151.88 - $708.08
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOB1
Gene ID: 28987
Uniprot ID: NOB1_HUMAN
Immunogen Region: 1-230
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NOB1 (NP_054781.1).
Immunogen Sequence: MAPVEHVVADAGAFLRHAAL QDIGKNIYTIREVVTEIRDK ATRRRLAVLPYELRFKEPLP EYVRLVTEFSKKTGDYPSLS ATDIQVLALTYQLEAEFVGV SHLKQEPQKVKVSSSIQHPE TPLHISGFHLPYKPKPPQET EKGHSACEPENLEFSSFMFW RNPLPNIDHELQELLIDRGE DVPSEEEEEEENGFEDRKDD SDDDGGGWITPSNIKQIQQE LEQCDVPEDV
Tissue Specificity Detected in liver, lung, placenta, endothelial cells and spleen.
Function May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits.
Protein Name Rna-Binding Protein Nob1
Phosphorylation Regulatory Protein Hp-10
Protein Art-4
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Rna-Binding Protein Nob1 antibody
Anti-Phosphorylation Regulatory Protein Hp-10 antibody
Anti-Protein Art-4 antibody
Anti-NOB1 antibody
Anti-ART4 antibody
Anti-NOB1P antibody
Anti-PSMD8BP1 antibody
Anti-MSTP158 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance